Today we planned to leave the hotel early again. And, we did. We left 30 minutes later than the plan was due to issues with my bank card, but overall, it was fine. 6:30 am, we were on the road.

Traffic in Lima didn’t slow us down as much as other yesterday. By 9:00 we had left the city and we had a break for a proper breakfast in a town of which I cannot remember the name. Shortly after this town the landscape changed dramatically, from dry Lima-suburban-slums, we suddenly found ourselves in a lush and agriculture rich area. No wild tomatoes though. So, we drove on. The road followed the river up into the mountains and by the time we reached 800 m altitude, the first wild tomatoes appeared, S. pimpinellifulium again. Today we were totally professional and all efficient, so we picked the plants, but did not process the samples immediately. We would collect a few sites and do all in batches. We found a very good site with dozens of S. pennellii in a bend in the road, going several hundred meters up. Here we collected additional samples and processed the previous ones as well. Not far from this site, we also found nice, but lonely S. peruvianum just on the side of the road.

After this plant we drove for quite a while. The occasional S. pennellii plant popped up, but we wanted to sample from a big population, so we could pick the best looking symptoms. I was just about to give up on finding one and wanted to suggest sampling a couple of these lonely plants next to the road, when Philippe yelled “Stop, stop, go back, go back”. I must have been looking tired or stupid, because he repeated himself a few more times. This, however, was a good thing, because after I reversed the car for a few hundred meters Philippe pointed me at the absolutely stunning site that I just overlooked. At least 50 plants growing in a dry river bed.

With plenty of S. pennellii in the pocket now, we were ready to drive into S. habrochaites country. The altitude meter told us we were passing 2000m, the area became greener and yes, there they were. Sometimes individually and sometimes in larger populations. We stopped at a site where we also found S. peruvianum and a third species which I think to be S corneliomulleri. I found very few P. infestans like symptoms on the S habrochaites. I expected this, because in the lab S. habrochaites is more resistant P. infestans than the other species we tested. Interestingly, there were some beautiful concentric rings with a yellow halo on quite a few leaves, so Alternria spp are probably doing fine on the plant. Next time, we should expand the collection permit. When I was about to head back, I suddenly heard some exciting scream behind me. Philippe had found a plant that showed some very clear and strong wilting symptoms. The hypothesis for this trip was that  the bacteria would be mostly asymptomatic in these species, so seeing this is quite exciting. However, we’ll have to wait till we’re back in the lab to know whether it is really bacterial wilt.

Early afternoon, we reached Canta. Our preferred hotel was closed, so we had to look for something else. We also still had to prep the samples of the second half of the morning morning. We did this in a hotel recommended by others, but we soon realised that there was no decent wifi connection and the beds were covered in dust. Motivated scientist as we are, that could have been OK, if only had we printed or at all prepared our route for tomorrow, so after sample prep in the dining room of the hotel and after having eaten our lunch there, we decided to move to another hotel.

The new hotel was only a few hundred meters away and looked much better. By the time we had checked in and settled down it turned out to be almost 15:00. Because our morning had been pretty successful and we both noticed some tiredness from the previous days, we decided to call it a day. This meant we finally had time to catch up with email and other work and also to just have a bit of a rest. For dinner we found a very typical Peruvian place, so we ordered Deep fried Guinnea Pig and a Pisco Sour at 2892 m above sea level. To not upset the readers of this blog, none of us took a picture of our meal, which wasn’t that tasty, by the way. Tomorrow we’re going “off road” to find more S habrochaites. I’m looking forward to it.

 

 

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